Translational_Unit
Part:BBa_K1362023:Design
Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha Group: iGEM14_Heidelberg (2014-10-08)
RBS + Xylanase (with His6)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 22
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 426
Illegal SapI.rc site found at 480
Design Notes
The His-Tag allows simple purification with His-Trap Column. Furthermore a Anti 6 His-Tag Antibody can be used on a Western Blot for detection of the enzyme.
Source
See the Basic Xylanase Part